Publications

@article{malivert2021turning,

title = {Turning plants from passive to active material: FERONIA and microtubules independently contribute to mechanical feedback},

author = {Alice Malivert and Ozer Erguvan and Antoine Chevallier and Antoine Dehem and Rodrique Friaud and Mengying Liu and Marjolaine Martin and Theophile Peyraud and Olivier Hamant and Stephane Verger},

year  = {2021},

date = {2021-01-01},

journal = {bioRxiv},

publisher = {Cold Spring Harbor Laboratory},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{kohorn2021effects,

title = {Effects of Arabidopsis wall associated kinase mutations on ESMERALDA1 and elicitor induced ROS},

author = {Bruce D Kohorn and Bridgid E Greed and Gregory Mouille and Stéphane Verger and Susan L Kohorn},

year  = {2021},

date = {2021-01-01},

journal = {Plos one},

volume = {16},

number = {5},

pages = {e0251922},

publisher = {Public Library of Science San Francisco, CA USA},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{malivert2021feronia,

title = {FERONIA and microtubules independently contribute to mechanical integrity in the Arabidopsis shoot},

author = {Alice Malivert and Özer Erguvan and Antoine Chevallier and Antoine Dehem and Rodrigue Friaud and Mengying Liu and Marjolaine Martin and Théophile Peyraud and Olivier Hamant and Stéphane Verger},

year  = {2021},

date = {2021-01-01},

journal = {PLoS biology},

volume = {19},

number = {11},

pages = {e3001454},

publisher = {Public Library of Science San Francisco, CA USA},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Johan_Henriksson_94038920,

title = {Mapping Rora expression in resting and activated CD4+ T cells},

author = {Johan Henriksson and Liora Haim-Vilmovsky and Scott N. Mueller and Johan Henriksson and Jennifer A. Walker and Zhichao Miao and Eviatar Natan and Gozde Kar and Simon Clare and Jillian L. Barlow and Evelina Charidemou and Lira Mamanova and Xi Chen and Valentina Proserpio and Jhuma Pramanik and Steven Woodhouse and Anna V. Protasio and Mirjana Efremova and Julian L. Griffin and Matt Berriman and Gordon Dougan and Jasmin Fisher and John C. Marioni and Andrew N. J. McKenzie and Sarah A. Teichmann},

url = {http://doi.org/10.1371/journal.pone.0251233},

doi = {10.1371/journal.pone.0251233},

year  = {2021},

date = {2021-01-01},

journal = {PLOS ONE},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Jafari_2021b,

title = {Activity and stress during a critical period regulate olfactory sensory neuron differentiation},

author = {Shadi Jafari and Johan Henriksson and Hua Yan and Mattias Alenius},

url = {https://doi.org/10.1101%2F2021.01.13.426514},

doi = {10.1101/2021.01.13.426514},

year  = {2021},

date = {2021-01-01},

publisher = {Cold Spring Harbor Laboratory},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Abreu2020,

title = {A metabolite roadmap of the wood-forming tissue in Populus tremula},

author = {Ilka N. Abreu and Annika I. Johansson and Katarzyna Sokołowska and Totte Niittylä and Björn Sundberg and Torgeir R. Hvidsten and Nathaniel R. Street and Thomas Moritz},

url = {https://onlinelibrary.wiley.com/doi/10.1111/nph.16799},

doi = {10.1111/nph.16799},

issn = {14698137},

year  = {2020},

date = {2020-12-01},

journal = {New Phytologist},

volume = {228},

number = {5},

pages = {1559--1572},

publisher = {Blackwell Publishing Ltd},

abstract = {Wood, or secondary xylem, is the product of xylogenesis, a developmental process that begins with the proliferation of cambial derivatives and ends with mature xylem fibers and vessels with lignified secondary cell walls. Fully mature xylem has undergone a series of cellular processes, including cell division, cell expansion, secondary wall formation, lignification and programmed cell death. A complex network of interactions between transcriptional regulators and signal transduction pathways controls wood formation. However, the role of metabolites during this developmental process has not been comprehensively characterized. To evaluate the role of metabolites during wood formation, we performed a high spatial resolution metabolomics study of the wood-forming zone of Populus tremula, including laser dissected aspen ray and fiber cells. We show that metabolites show specific patterns within the wood-forming zone, following the differentiation process from cell division to cell death. The data from profiled laser dissected aspen ray and fiber cells suggests that these two cell types host distinctly different metabolic processes. Furthermore, by integrating previously published transcriptomic and proteomic profiles generated from the same trees, we provide an integrative picture of molecular processes, for example, deamination of phenylalanine during lignification is of critical importance for nitrogen metabolism during wood formation.},

keywords = {cambium, crysectioning, laser capture microdissection, metabolomics, Populus, wood},

pubstate = {published},

tppubtype = {article}

}

@article{Mahler2020,

title = {Leaf shape in Populus tremula is a complex, omnigenic trait},

author = {Niklas Mähler and Bastian Schiffthaler and Kathryn M. Robinson and Barbara K. Terebieniec and Matej Vučak and Chanaka Mannapperuma and Mark E. S. S. Bailey and Stefan Jansson and Torgeir R. Hvidsten and Nathaniel R. Street},

url = {https://onlinelibrary.wiley.com/doi/full/10.1002/ece3.6691 https://onlinelibrary.wiley.com/doi/abs/10.1002/ece3.6691 https://onlinelibrary.wiley.com/doi/10.1002/ece3.6691},

doi = {10.1002/ece3.6691},

issn = {2045-7758},

year  = {2020},

date = {2020-11-01},

journal = {Ecology and Evolution},

volume = {10},

number = {21},

pages = {11922--11940},

publisher = {John Wiley and Sons Ltd},

keywords = {complex trait, GWAS, leaf shape, natural variation, omnigenic, Populus tremula},

pubstate = {published},

tppubtype = {article}

}

@article{PhysRevE.102.012302,

title = {Mapping flows on sparse networks with missing links},

author = {Jelena Smiljanic and Daniel Edler and Martin Rosvall},

url = {https://link.aps.org/doi/10.1103/PhysRevE.102.012302},

doi = {10.1103/PhysRevE.102.012302},

year  = {2020},

date = {2020-07-01},

journal = {Phys. Rev. E},

volume = {102},

pages = {012302},

publisher = {American Physical Society},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid32634386,

title = {How geographic productivity patterns affect food-web evolution},

author = {Jonas Wickman and Ulf Dieckmann and Cang Hui and Åke Brännström},

year  = {2020},

date = {2020-07-01},

journal = {J. Theor. Biol.},

volume = {506},

pages = {110374},

note = {[DOI:hrefhttps://dx.doi.org/10.1016/j.jtbi.2020.11037410.1016/j.jtbi.2020.110374] [PubMed:hrefhttps://www.ncbi.nlm.nih.gov/pubmed/3263438632634386]},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Muller2020,

title = {A single gene underlies the dynamic evolution of poplar sex determination},

author = {Niels A. Müller and Birgit Kersten and Ana P. Leite Montalvão and Niklas Mähler and Carolina Bernhardsson and Katharina Bräutigam and Zulema Carracedo Lorenzo and Hans Hoenicka and Vikash Kumar and Malte Mader and Birte Pakull and Kathryn M. Robinson and Maurizio Sabatti and Cristina Vettori and Pär K. Ingvarsson and Quentin Cronk and Nathaniel R. Street and Matthias Fladung},

url = {http://www.nature.com/articles/s41477-020-0672-9},

doi = {10.1038/s41477-020-0672-9},

issn = {2055-0278},

year  = {2020},

date = {2020-06-01},

journal = {Nature Plants},

pages = {1--8},

publisher = {Nature Publishing Group},

abstract = {Although hundreds of plant lineages have independently evolved dioecy (that is, separation of the sexes), the underlying genetic basis remains largely elusive1. Here we show that diverse poplar species carry partial duplicates of the ARABIDOPSIS RESPONSE REGULATOR 17 (ARR17) orthologue in the male-specific region of the Y chromosome. These duplicates give rise to small RNAs apparently causing male-specific DNA methylation and silencing of the ARR17 gene. CRISPR–Cas9-induced mutations demonstrate that ARR17 functions as a sex switch, triggering female development when on and male development when off. Despite repeated turnover events, including a transition from the XY system to a ZW system, the sex-specific regulation of ARR17 is conserved across the poplar genus and probably beyond. Our data reveal how a single-gene-based mechanism of dioecy can enable highly dynamic sex-linked regions and contribute to maintaining recombination and integrity of sex chromosomes. Populus has young sex chromosomes despite ancient dioecy. This study shows that the ARR17 gene functions as a sex switch, triggering female development when on and male development when off. This single-gene system enables dynamic evolution of poplar sex chromosomes.},

keywords = {evolution, Genomics, Natural variation in plants, Plant evolution, Plant genetics},

pubstate = {published},

tppubtype = {article}

}

@article{pmid32547784,

title = {Ecological speciation in European whitefish is driven by a large-gaped predator},

author = {Gunnar Öhlund and Mats Bodin and Karin A. Nilsson and Sven‐Ola Öhlund and Kenyon B. Mobley and Alan G. Hudson and Mikael Peedu and Åke Brännström and Pia Bartels and Kim Præbel and Catherine L. Hein and Petter Johansson and Göran Englund},

year  = {2020},

date = {2020-06-01},

journal = {Evol Lett},

volume = {4},

number = {3},

pages = {243--256},

note = {[PubMed Central:hrefhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7293097PMC7293097] [DOI:hrefhttps://dx.doi.org/10.1002/evl3.16710.1002/evl3.167] [PubMed:hrefhttps://www.ncbi.nlm.nih.gov/pubmed/2665578226655782]},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Enevold2020,

title = {Tunable Two-Dimensional Patterning of a Semiconducting and Nanometer-Thin C 60 Fullerene Film Using a Spatial Light Modulator},

author = {Jenny Enevold and Tobias Dahlberg and Tim Stangner and Shi Tang and E. Mattias Lindh and Eduardo Gracia-Espino and Magnus Andersson and Ludvig Edman},

url = {https://pubs.acs.org/doi/10.1021/acsanm.0c00793},

doi = {10.1021/acsanm.0c00793},

issn = {2574-0970},

year  = {2020},

date = {2020-05-01},

journal = {ACS Applied Nano Materials},

pages = {acsanm.0c00793},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Escamez2020,

title = {Cell Death in Cells Overlying Lateral Root Primordia Facilitates Organ Growth in Arabidopsis},

author = {Sacha Escamez and Domenique André and Bernadette Sztojka and Benjamin Bollhöner and Hardy Hall and Béatrice Berthet and Ute Voß and Amnon Lers and Alexis Maizel and Magnus Andersson and Malcolm Bennett and Hannele Tuominen},

url = {https://linkinghub.elsevier.com/retrieve/pii/S0960982219315805},

doi = {10.1016/j.cub.2019.11.078},

issn = {09609822},

year  = {2020},

date = {2020-02-01},

journal = {Current Biology},

volume = {30},

number = {3},

pages = {455--464.e7},

abstract = {Unlike animal development, plant organ growth is widely accepted to be determined by cell division without any contribution of cell elimination. We investigated this paradigm during Arabidopsis lateral root formation when growth of the new primordia (LRP) from pericycle-derived stem cells deep inside the root is reportedly facilitated by remodeling of the walls of overlying cells without apparent cell death. However, we observed the induction of marker genes for cell types undergoing developmental cell death in several cells overlying the growing LRP. Transmission electron microscopy, time-lapse confocal and light sheet microscopy techniques were used to establish that cell death occurred at least in a subset of endodermal LRP-overlying cells during organ emergence. Significantly, organ emergence was retarded in mutants lacking a positive cell death regulator, and restored by inducing cell death in cells overlying LRP. Hence, we conclude that in the case of LRP, cell elimination contributes to organ growth.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Haas2020,

title = {Candidate regulators and target genes of drought stress in needles and roots of Norway spruce. },

author = {Julia C. Haas and Alexander Vergara and Vaughan Hurry and Nathaniel R. Street.},

doi = {10.1101/517151},

year  = {2020},

date = {2020-01-20},

journal = {Biorxiv},

keywords = {vergara},

pubstate = {published},

tppubtype = {article}

}

@article{Vergara2020,

title = { Norway spruce deploys tissue specific canonical responses to acclimate to cold.  },

author = {Alexander Vergara and Julia Christa Haas and Paulina Stachula and Nathaniel R. Street and Vaughan Hurry},

doi = {10.1101/2020.01.13.904805},

year  = {2020},

date = {2020-01-17},

journal = {Biorxiv},

keywords = {vergara},

pubstate = {published},

tppubtype = {article}

}

@article{mahmud2020prokseq,

title = {ProkSeq for complete analysis of RNA-seq data from prokaryotes},

author = {AKM Firoj Mahmud and Soumyadeep Nandi and Maria Fallman},

year  = {2020},

date = {2020-01-01},

journal = {bioRxiv},

publisher = {Cold Spring Harbor Laboratory},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Mahmude01006-20,

title = {Genome-Scale Mapping Reveals Complex Regulatory Activities of RpoN in Yersinia pseudotuberculosis},

author = {Firoj A K M Mahmud and Kristina Nilsson and Anna Fahlgren and Roberto Navais and Rajdeep Choudhury and Kemal Avican and Maria Fällman},

editor = {Ryan McClure},

url = {https://msystems.asm.org/content/5/6/e01006-20},

doi = {10.1128/mSystems.01006-20},

year  = {2020},

date = {2020-01-01},

journal = {mSystems},

volume = {5},

number = {6},

publisher = {American Society for Microbiology Journals},

abstract = {RpoN, an alternative sigma factor commonly known as σ54, is implicated in persistent stages of Yersinia pseudotuberculosis infections in which genes associated with this regulator are upregulated. We here combined phenotypic and genomic assays to provide insight into its role and function in this pathogen. RpoN was found essential for Y. pseudotuberculosis virulence in mice, and in vitro functional assays showed that it controls biofilm formation and motility. Mapping genome-wide associations of Y. pseudotuberculosis RpoN using chromatin immunoprecipitation coupled with next-generation sequencing identified an RpoN binding motif located at 103 inter- and intragenic sites on both sense and antisense strands. Deletion of rpoN had a large impact on gene expression, including downregulation of genes encoding proteins involved in flagellar assembly, chemotaxis, and quorum sensing. There were also clear indications of cross talk with other sigma factors, together with indirect effects due to altered expression of other regulators. Matching differential gene expression with locations of the binding sites implicated around 130 genes or operons potentially activated or repressed by RpoN. Mutagenesis of selected intergenic binding sites confirmed both positive and negative regulatory effects of RpoN binding. Corresponding mutations of intragenic sense sites had less impact on associated gene expression. Surprisingly, mutating intragenic sites on the antisense strand commonly reduced expression of genes carried by the corresponding sense strand.IMPORTANCE The alternative sigma factor RpoN (σ54), which is widely distributed in eubacteria, has been implicated in controlling gene expression of importance for numerous functions including virulence. Proper responses to host environments are crucial for bacteria to establish infection, and regulatory mechanisms involved are therefore of high interest for development of future therapeutics. Little is known about the function of RpoN in the intestinal pathogen Y. pseudotuberculosis, and we therefore investigated its regulatory role in this pathogen. This regulator was indeed found to be critical for establishment of infection in mice, likely involving its requirement for motility and biofilm formation. The RpoN regulon involved both activating and suppressive effects on gene expression which could be confirmed with mutagenesis of identified binding sites. This is the first study of its kind of RpoN in Y. pseudotuberculosis, revealing complex regulation of gene expression involving both productive and silent effects of its binding to DNA, providing important information about RpoN regulation in enterobacteria.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Avican2020.06.29.177147,

title = {RNA Atlas of Human Bacterial Pathogens Uncovers Stress Dynamics Linked to Infection},

author = {Kemal Avican and Jehad Aldahdooh and Matteo Togninalli and Jing Tang and Karsten M Borgwardt and Mikael Rhen and Maria Fällman},

url = {https://www.biorxiv.org/content/early/2020/06/29/2020.06.29.177147},

doi = {10.1101/2020.06.29.177147},

year  = {2020},

date = {2020-01-01},

journal = {bioRxiv},

publisher = {Cold Spring Harbor Laboratory},

abstract = {Despite being genetically diverse, bacterial pathogens can adapt to similar stressful environments in human host, but how this diversity allows them to achive this is yet not fully understood. Knowledge gained through comparative genomics is insufficient as it lacks the level of gene expression reflecting gene usage. To fill this gap, we investigated the transcriptome of 32 diverse bacterial pathogens under 11 host related stress conditions. We revealed that diverse bacterial pathogens have common responses to similar stresses to a certain extent but mostly employ their unique repertoire with intersections between different stress responses. We also identified universal stress responders which shed light on the nature of antimicrobial targets. In addition, we found that known and unknown putative novel ncRNAs comprised a significant proportion of the responses. All the data is collected in PATHOgenex atlas, providing ample opportunities to discover novel players critical for virulence and maintenance of infection.Competing Interest StatementThe authors have declared no competing interest.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Schneiders2020.11.04.369199,

title = {Spatiotemporal Variations in Growth Rate and Virulence Plasmid Copy Number during Yersinia pseudotuberculosis Infection},

author = {Stephan Schneiders and Tifaine Hechard and Tomas Edgren and Kemal Avican and Maria Fällman and Anna Fahlgren and Helen Wang},

url = {https://www.biorxiv.org/content/early/2020/11/06/2020.11.04.369199},

doi = {10.1101/2020.11.04.369199},

year  = {2020},

date = {2020-01-01},

journal = {bioRxiv},

publisher = {Cold Spring Harbor Laboratory},

abstract = {Pathogenic Yersinia spp. depend on the activity of a potent virulence plasmid-encoded ysc/yop type 3 secretion system (T3SS) to colonize hosts and cause disease. It was recently shown that Y. pseudotuberculosis up-regulates the virulence plasmid copy number (PCN) during infection and the resulting elevated gene dose of plasmid-encoded T3SS genes is essential for virulence. When and how this novel regulatory mechanism is deployed and regulates the replication of the virulence plasmid during infection is unknown. In the current study, we applied droplet digital PCR (ddPCR) to investigate the dynamics of Y. pseudotuberculosis virulence PCN variations and growth rates in infected mouse organs. We demonstrated that both PCN and growth varied in different tissues and over time throughout the course of infection, indicating that the bacteria adapted to discrete microenvironments during infection. The PCN was highest in Peyertextquoterights Patches and caecum during the clonal invasive phase of the infection, while the fastest growth rates were found in the draining mesenteric lymph nodes. In deeper, systemic organs, the PCN was lower and more modest growth rates were recorded. Our study indicates that increased gene dosage of the plasmid-encoded T3SS genes is most important early in the infection during invasion of the host. The described ddPCR approach will greatly simplify analyses of PCN, growth dynamics, and bacterial loads in infected tissues, and will be readily applicable to other infection models.Importance Studying pathogenic bacteria proliferating inside infected hosts is challenging using traditional methods, especially the transit and reversible genetic events. The bacteria are effectively diluted by the overwhelming number of host cells present in infected tissues. Using an innovative droplet digital PCR (ddPCR) approach, we have determined the virulence plasmid copy number (PCN) variations and growth rates of Yersinia during the course of infection in a mouse model. Here, we show that both the virulence plasmid copy number and bacterial growth rates display spatiotemporal variations in mice during infection. We demonstrate that the peak-to-trough ratio can be used as a proxy for determining the growth rate of invasive bacterial pathogen during infection, and ddPCR as the method of choice for quantifying DNA in host-pathogen interaction context. This proof-of-concept ddPCR approach can be easily applied for any bacterial pathogens and any infection models, for analysis of PCN, growth dynamics and bacterial loads.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{fernandez2020non,

title = {Non-cyanobacterial diazotrophs dominate nitrogen-fixing communities in permafrost thaw ponds},

author = {Leyden Fernandez and Stefan Bertilsson and Sari Peura},

year  = {2020},

date = {2020-01-01},

journal = {Limnology and Oceanography},

volume = {65},

pages = {S180--S193},

publisher = {John Wiley & Sons, Inc. Hoboken, USA},

keywords = {},

pubstate = {published},

tppubtype = {article}

}